Macromolecular Cristallography at Sirius

• ACM Zeri, AFN Ziem, C Hagio, and LNLS/CNPEM

• Brazilian Synchrotron Source, Campinas – LNLS/CNPEM, Brazil

• ana.zeri@lnls.br

Sirius is the new Brazilian Synchrotron light source, under construction in Campinas, São Paulo state. It will be a 4th generation source, with various applications in Biophysics. Advanced imaging techniques will be available alongside new in-operando techniques. The MANACA beamline will be installed in a high-beta straight section of the Sirius storage ring and its main goal is to bridge the gap between conventional crystallography, which uses large single crystals, and single-molecule X-ray diffraction performed in free electron lasers. The beamline will have two experimental stations, for the performance of state-of-the-art macromolecular micro-crystallography employing current methods, with a beam focused in the low tens of micrometers, and nano-crystallography, with a submicron focus beam and sub miliradian divergence. The beamline will operate with X-ray energies between 5 to 20 keV with large emphasis on membrane proteins and supra-macromolecular protein complexes (megadalton size), such as receptors, proteasomes, translation complexes (protein and DNA/RNA components), bacterial cell division machinery and eukaryotic transcription complexes, and will enable a range of automated screening assays for molecular fragments and natural products. The applications cover areas such as pharmaceutics, agriculture and cosmetics, and will enable the use and development of new techniques for the investigation of biological materials at the atomic level.