Magnetic carbon nanotubes modified with proteins and hydrophilic monomers: Cytocompatibility, in-vitro toxicity assays and permeation across biological interfaces
Abstract: Carbon nanotubes are promising materials for biomedical applications like delivery systems and tissue scaffolds. In this paper, magnetic carbon nanotubes (M-CNTs) covered with bovine serum albumin (M-CNTs-BSA) or functionalized with hydrophilic monomers (M-CNTs-HL) were synthesized, characterized, and evaluated concerning their interaction with Caco-2 cells. There is no comparison between these two types of functionalization, and this study aimed to verify their influence on the material’s interaction with the cells. Different concentrations of the nanotubes were applied to investigate cytotoxicity, cell metabolism, oxidative stress, apoptosis, and capability to cross biomimetic barriers. The materials showed cytocompatibility up to 100 μg mL−1 and a hemolysis rate below 2 %. Nanotubes’ suspensions were allowed to permeate Caco-2 monolayers for up to 8 h under the effect of the magnetic field. Magnetic nanoparticles associated with the nanotubes allowed estimation of permeation through the monolayers, with values ranging from 0.50 to 7.19 and 0.27 to 9.30 × 10−3 μg (equivalent to 0.43 to 6.22 and 0.23 to 9.54 × 10−2 % of the initially estimated mass of magnetic nanoparticles) for cells exposed and non-exposed to the magnets, respectively. Together, these results support that the developed materials are promising for applications in biomedical and biotechnological fields.
Author(s): Mariana Azevedo Rosa, Andreia Granja, Cláudia Nunes, Salette Reis, Ana Beatriz Santos da Silva, Ketolly Natanne da Silva Leal, Marco Aurélio Zezzi Arruda, Luiz Fernando Gorup, Mariane Gonçalves Santos, Marcos Vinicios Salles Dias, Eduardo Costa Figueiredo.
International Journal of Biological Macromolecules
Published: June 2024, Volume 269, Part 1, 131962
DOI: https://doi.org/10.1016/j.ijbiomac.2024.131962
CDMF
The CDMF, hosted at the Federal University of São Carlos (UFSCar), is one of the Research, Innovation and Dissemination Centers (RIDC) supported by the São Paulo State Research Support Foundation (Fapesp), and also receives investment from the National Council Scientific and Technological Development (CNPq), from the National Institute of Science and Technology of Materials in Nanotechnology (INCTMN).
