Siloxane-modified bacterial cellulose as a promising platform for cell culture
Abstract: Cellulose is a versatile tunable material that finds application in the biomedical field as substrate for cell culture. However, few studies have been done to explore the use of bacterial cellulose (BC), a naturally occurring nanofibrillar material with distinct properties, as a platform to produce such device. In the present work, BC membranes have been functionalized with thiol functional group (SH) through silanization reaction with (3-mercaptopropyl)trimethoxysilane (MPTMS) under different conditions, in order to obtain a platform with improved cell adhesion. The efficiency of BC surface modification with MPTMS was evaluated by using acid and base catalyzed reactions, and two different drying methods: at room temperature of 28 °C and at 120 °C as curing temperature. The results of the set of analyses performed—ATR-FTIR, TGA, elemental analysis,13C NMR, contact angle and SEM—indicate that BC surface functionalization was efficient, regardless the drying process. The MPTMS-modified platforms exhibited sulfur content of 3–5 times higher than native BC. The performed biological assay with fibroblast GM07492 human cells revealed that adhesion of cells to the BC surface depends not only on the functional group present at the matrix but also on surface wettability.
Author(s): Claro, A. M.; Do Amaral, N. C.; Medalha Colturato, V. M.; Aleixo, N. A.; Paiva, R.; Cruz, S. A.; Monteiro, G. C.; Goncalves De Carvalho, G. S.; Resende Nogueira, F. A.; Deffune, E.; da Costa Iemma, M. R.; Barud, H. d. S.
Cellulose
Published: 16 October 2022
DOI: https://doi.org/10.1007/s10570-022-04872-4
CDMF
The CDMF, hosted at the Federal University of São Carlos (UFSCar), is one of the Research, Innovation and Dissemination Centers (RIDC) supported by the São Paulo State Research Support Foundation (Fapesp), and also receives investment from the National Council Scientific and Technological Development (CNPq), from the National Institute of Science and Technology of Materials in Nanotechnology (INCTMN).
