Photodynamic inactivation of clinical isolates of Candida using Photodithazine®

Biofouling Volume: 29 Issue: 9 Pages: 1057-1067 Published: 2013

Summary of log CFU ml-1 (A) and XTT (B) values obtained after evaluation of the PDI groups of planktonic and biofilms cultures of C. albicans, respectively. The box plots use the median (bold square), the first and third quartiles (box), and the lowest and highest values (dashes). The same lower case letter denotes non-significant differences among groups according to nonparametric post hoc test (p > 0.05).

Writers: L.N. Dovigo; J.C. Carmello; M.T. Carvalho; E.G. Mima; C.E. Vergani; V.S. Bagnato & A.C. Pavarina

Keywords: photodynamic therapy; Candida spp.; biofilms

Abstract: This study evaluated the photodynamic inactivation (PDI) mediated by Photodithazine® (PDZ) against 15 clinical isolates of Candida albicans, Candida glabrata and Candida tropicalis. Each isolate, in planktonic and biofilm form, was exposed to PDI by assessing a range of PDZ concentrations and light emitting diode fluences. Cell survival of the planktonic suspensions was determined by colony forming units (CFU ml−1). The antifungal effects of PDI against biofilms were evaluated by CFU ml−1 and metabolic assay. Data were analyzed by non-parametric tests (α = 0.05). Regardless of the species, PDI promoted a significant viability reduction of planktonic yeasts. The highest reduction in cell viability of the biofilms was equivalent to 0.9 log10 (CFU ml−1) for C. albicans, while 1.4 and 1.5 log10 reductions were obtained for C. tropicalis and C. glabrata, respectively. PDI reduced the metabolic activity of biofilms by 62.1, 76.0, and 76.9% for C. albicans, C. tropicalis, and C. glabrata, respectively. PDZ-mediated PDI promoted significant reduction in the viability of Candida isolates.

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DOI: 10.1080/08927014.2013.827668

Robson Lima
Sobre Robson Lima 87 Artigos
Atua no Centro de Desenvolvimento de Materiais Funcionais.