Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth

Journal of Microbiological Methods Volume: 125 Pages: 40-42 Published: 2016

Images obtained by inverted microscope for the DMEM, RPMI/HEPES pH 7.0, RPMI/HEPES pH 8.2 and RPMI/MOPS with 12 h of incubation in NOK and HaCat cell groups. Magnification ×20.

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Writers: Kássia de Carvalho Dias and Paula Aboud Barbugli and Carlos Eduardo Vergani

Keywords: Cell survival; Keratinocytes; HEPES; Candida albicans; Staphylococcus aureus

Abstract: This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.

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