In vitro evaluation of the enzymatic activity profile of non-albicans Candida species isolated from patients with oral candidiasis with or without diabetes

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Volume: 118 Issue: 1 Pages: 84-91 Published: 2014

Factorial analyses of variance for the enzymatic activity data

Writers: Paula V. Sanitá, Chaiene E. Zago, Ewerton G. de O. Mima, Ana C. Pavarina, Janaina H. Jorge, Ana L. Machado, Carlos E. Vergani.

Abstract: Objective To evaluate the expression of phospholipase (PL) and secreted aspartyl proteinase (SAP) by Candida glabrata and C tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates).

Study Design: After incubation, the supernatants and pellets of the isolates were used for the enzymatic assays and quantification of colony-forming units (CFU), respectively. Colorimetric tests were used with phosphatidylcholine as a substrate for PL and azocasein as a substrate for SAP, and the absorbances of the samples were measured. Enzymatic rates were calculated, and values were normalized by CFU. Results were analyzed with factorial analyses of variance (α = .05).

Results: C tropicalis and C glabrata were proteolytic and phospholipolytic. The clinical sources of isolates had no significant effect on the enzymatic activities (P > .05). C tropicalis had significantly higher enzymatic activity for both PL and SAP (P < .001) than did C glabrata.

Conclusions: C tropicalis isolates produced significantly higher amounts of both enzymes than did the C glabrata isolates.

DOI: 10.1016/j.oooo.2014.03.020

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